Use of an enzyme-linked immunoassay for Clostridium difficile serogrouping
نویسندگان
چکیده
منابع مشابه
Evaluation of Correlation between Pretest Probability for Clostridium difficile Infection and Clostridium difficile Enzyme Immunoassay Results.
The objective of this study was to evaluate the clinical characteristics and outcomes of hospitalized patients tested for Clostridium difficile and determine the correlation between pretest probability for C. difficile infection (CDI) and assay results. Patients with testing ordered for C. difficile were enrolled and assigned a high, medium, or low pretest probability of CDI based on clinical e...
متن کاملSerogrouping of Clostridium difficile strains by slide agglutination.
Six different agglutinating antisera were obtained by immunizing rabbits with Formol-treated strains of Clostridium difficile. After appropriate absorption, these antisera were used to define six serogroups designated by the letters A, B, C, D, F, and G. Altogether, 315 strains of C. difficile from various origins were tested for slide agglutination by these antisera; 312 (99%) of them were agg...
متن کاملNonutility of repeat laboratory testing for detection of Clostridium difficile by use of PCR or enzyme immunoassay.
The diagnostic gains of repeat testing for Clostridium difficile by enzyme immunoassay and PCR (i.e., initial negative result followed by positive result) within a 7-day period were 1.9 and 1.7%, respectively. There is little value of repeat testing for C. difficile by enzyme immunoassay or PCR.
متن کاملEvaluation of a new enzyme immunoassay for Clostridium difficile toxin A.
AIM To evaluate a new enzyme immunoassay (EIA) method for detection of Clostridium difficile toxin by comparing it to cytotoxicity assay. To investigate the nature of false negative and false positive EIA results by evaluating clinical and therapeutic parameters. METHODS 737 consecutive diarrhoeal specimens collected from patients clinically suspected of having C difficile colitis were tested...
متن کاملEvaluation of an Enzyme Immunoassay for Detection of the Clostridium difficile Common Antigen, Glutamate Dehydrogenase (GDH), in Fecal Specimens
C. difficile is reported to cause up to 25% of cases of antibiotic-associated diarrhea (AAD) and most cases of pseudomembranous colitis (PMC). Laboratory diagnosis typically consists of toxin detection in fecal specimens by enzyme immunoassay (EIA) or tissue culture cytotoxin assay (CTA). Culture on C. difficile selective media offers the highest sensitivity, but has low specificity due to dete...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1993
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.31.9.2526-2528.1993